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Diagnosis of Swine Flu

Patients with flu-like-symptoms should seek medical help immediately on onset of symptoms. This helps in early diagnosis, proper treatment and prevention of complications. For diagnosis of swine influenza A infection, respiratory specimen (NP (nasopharyngeal swab), throat swab (From TOI - 17/08/2009), nasal aspirate, nasal washing) would generally need to be collected within the first 4 to 5 days of illness (when an infected person is most likely to be shedding virus). However, some persons, especially children, may shed virus for 10 days or longer.

Sample Collection & Laboratory Diagnosis
  • Sample Collection and handling is same as for human avian flu or seasonal influenza like illness (Refer CD Alert on AI).
  • Sample Collection: should be labeled clearly and include patient’s complete information and should be sent to NIV, Pune or NICD, Delhi within 24 hours for further investigations.
  • Laboratory biosafety measures should be followed for collection, storage, packaging and shipping of influenza samples.
Available Laboratory tests:
  • Rapid Antigen Tests: not as sensitive as other available tests.
  • RT–PCR
  • Virus isolation
  • Virus Genome Sequencing
  • Four–fold rise in swine influenza A (H1N1) virus specific neutralizing antibodies. It is important to note that samples from all cases, once the Pandemic starts, are not required to be tested.

Important Contact Numbers: Outbreak Monitoring Cell (Control Room, NICD):
Phone No: +91 11 23921401
EMR Control room (Ministry of health and family Welfare:
Phone No: +91 11 23061469
Important Websites:
www.mohfw.nic.in
www.nicd.nic.in
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